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Image Search Results
Journal: bioRxiv
Article Title: Cold Tolerance is Governed by Diverse Genetic Mechanisms Including Those Regulated by NB-LRR-type Receptor Proteins in Arabidopsis
doi: 10.1101/2022.01.18.476799
Figure Lengend Snippet: Schematic representation of the steps taken in identifying genes involved in cold stress using a high-throughput phenotyping platform. After suspending in low melting agarose (a) , seeds were stratified, and sown (b), and grown in the Arabidopsis growth chambers fitted with digital cameras (c) . Two-dimensional images of the rosette leaves of individual genotypes were captured and analyzed using Matlab GUI software (Method S2) (d) . The digital images were converted to pixel data for conducting easyGWAS to identify putative cold-stress-related genes. The candidate cold-stress-related genes were validated by studying knockout mutants. Homozygous knockout mutants were identified (e) . SALK_104944, knockout T-DNA insertion mutant for AT1G68320 ; HM, homozygous line; HZ, heterozygous line; WT (Col-0 ecotype), wild-type with no insertion. Knockout mutants were phenotyped to determine if any of the 33 putative cold-stress-related genes play a role in cold tolerance (f) .
Article Snippet: Two-dimensional images of the rosette leaves of individual mutants were captured and analyzed by
Techniques: High Throughput Screening Assay, Software, Knock-Out, Mutagenesis
Journal: Frontiers in Neuroscience
Article Title: MRIES: A Matlab Toolbox for Mapping the Responses to Intracranial Electrical Stimulation
doi: 10.3389/fnins.2021.652841
Figure Lengend Snippet: Main GUI of the toolbox. (A) Data processing GUI, including the processing pipeline, separate processing step, and visualization. (B) Visualization GUI. The individual brain is shown in different views. The lower right part shows the information and controllers highlighted in the color-coded box. The volume and voxel coordinates are illustrated in the blue box. The subject information is illustrated in the pink box. The electrode information is illustrated in the yellow box. The specific stimulated electrode pair and indicator illustrated in the green box can be chosen and then the responses to this electrode pair are shown in the volume space. Other visualization GUIs and 3D-electrode locations are shown in the red box.
Article Snippet: The set of the tools are easily available by
Techniques:
Journal: Cell reports
Article Title: D1 and D2 neurons in the nucleus accumbens enable positive and negative control over sugar intake in mice
doi: 10.1016/j.celrep.2023.112190
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit-anti-mCherry Abcam RRID: AB_2571870 TRITC-conjugated affinipure Goat anti-Rabbit IgG (H + L) Jackson ImmunoResearch Labs RRID: AB_2337932 Goat Anti-GFP antibody (FITC) Abcam RRID: AB_305635 Anti-c-Fos Rabbit polyclonal to c-Fos Abcam RRID: AB_2231989 Biotinylated Goat Anti-Rabbit IgG antibody Vector Laboratories RRID: AB_2313606 Chemicals, peptides, and recombinant proteins Sucralose Sigma PubChem Substance ID: 329769983 Rebaudioside A Sigma PubChem Substance ID: 329747271 Glucose Sigma PubChem Substance ID: 24895335 Methyl α-D-glucopyranoside Sigma PubChem Substance ID: 24897328 Denatonium Benzoate Sigma PubChem Substance ID: 57654112 Lithium chloride Sigma PubChem Substance ID: 24896459 Victoza (liraglutide) injection Novo Nordisk N/A DiI lipophiliccarbocyanine dye Sigma PubChem Substance ID: 57650110 PubChem Substance ID: Invitrogen Cat #D1817 Dextran, Tetramethylrhodamine, 10,000 MW, Lysine Fixable (Fluoro-Ruby) Critical commercial assays VECTASTAIN Elite ABC HRP Kit (Peroxidase, Standard) Vector Laboratories Cat# PK-6100 C&B Metabond Parkell SKU: S380 Experimental models: Organisms/strains Mouse: B6.FVB(Cg)-Tg(Drd1-cre)EY262Gsat/Mmucd University of California, Davis MMRRC RRID:MMRRC_030989-UCD Mouse: B6.FVB(Cg)-Tg(Drd2-cre)ER44Gsat/Mmucd University of California, Davis MMRRC RRID:MMRRC_032108-UCD Mouse: B6(Cg)-Tg(Phox2b-cre)3Jke/J The Jackson Laboratory RRID:IMSR_JAX:016223 Mouse: B6; 129S6-Gt(ROSA)26Sortm9 (CAG-tdTomato)Hze/J The Jackson Laboratory RRID:IMSR_JAX:007905 Recombinant DNA and virus strains H129 CAG-LSL-TK-tdTomato NIH Center for Neuroanatomy with Neurotropic Virus ΔTK-TT PRV CMV-EGFP NIH Center for Neuroanatomy with Neurotropic Virus Ba-152 PRV CAG-DIO-TK-GFP NIH Center for Neuroanatomy with Neurotropic Virus Ba-2017 AAV-flex-taCasp3-TEVp Addgene Cat # 45580-AAV5; RRID:Addgene_45580 AAV-EF1a-DIO-hChR2(H134R)-EYFP Addgene Cat # 20298-AAV5; RRID:Addgene_20298 Software and
Techniques: Plasmid Preparation, Recombinant, Injection, Software